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Pathological and biochemical omics analysis

Aniphe BioLab offers an integrated biomedical testing and mechanistic analysis platform that encompasses “pathological analysis, physicochemical assays, and multi-omics profiling,” serving basic research, translational medicine, and drug‑efficacy evaluation. The platform establishes a comprehensive data pipeline, spanning from tissue‑level structural changes to molecular‑level mechanisms.

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Pathological Analysis

In the realm of pathological analysis, the platform offers standardized sample‑processing workflows for paraffin‑embedded, frozen, and OCT‑embedded specimens, as well as decalcification of bone tissue. It supports a wide array of histological staining techniques, including H&E, Masson’s trichrome, PAS, Sirius Red, and TRAP. Complemented by TUNEL‑mediated apoptosis assays, immunohistochemistry and immunofluorescence, multiplexed TSA labeling, and whole‑slide scanning and imaging, the platform enables high‑precision, visualized, and quantitative analyses of tissue morphology and protein expression, thereby significantly enhancing the accuracy and reproducibility of pathological findings.

Experiment Name	Content	Explanation
Pathological section	Decalcification of bone tissue	Includes: slow release, fast release
	Paraffin embedding
	OCT embedding
	Paraffin section	Standard Adhesion Slides
	Frozen section
Pathological staining	H&E staining	Animal Tissue Morphology and Structure
	Pathological staining	Various stains, including Masson, PAS, Sirius red, and TRAP
	TUNEL apoptosis assay	There are two types: brightfield and fluorescence.
Immunohistochemistry	Routine immunohistochemistry	DAB staining, Fast Red staining
	Indirect fluorescence assay	Multi-label
	Immunofluorescence - Construction of the TSA Fluorescence Assay Protocol (4–6 Labels)
Pathological imaging scan	Photography (brightfield, local field of view) - 10X	Each slide provides 8 random fields of view.
	Whole-slide scanning (brightfield) – 20X
	Immunofluorescence Whole-Slide Scanning – 20X	Single-label and multi-label
	Pathomorphological description

Physiological and biochemical testing

In the realm of physiological and biochemical assays, the platform has established a standardized testing workflow that encompasses a wide array of parameters, including complete blood count, urinalysis, coagulation profile, blood gas and electrolyte analysis, and liver function tests. This comprehensive approach enables systematic assessment of the body’s overall physiological status and dynamic disease‑related changes, thereby providing robust data to support model validation and pharmacological efficacy evaluation.

Experiment Name	Content	Explanation
Blood and urine biochemical analysis	Coagulation Panel (4 Items)	PT, TT, Fib, APTT
	Complete Blood Gas and Electrolyte Analysis	Mg, K, Na, Cl, Ca, P, Zn, LAC, U-TP
	Complete blood count test	Hematology analyzer
	Urinalysis	Uri-Trak Urine Analyzer
	Liver function test	ALT, AST, DBil, IBil, TBil

Omics testing

At the omics analysis level, we integrate cutting-edge technologies—including metabolomics, proteomics, transcriptomic sequencing, single-cell sequencing, and metagenomic analysis—to enable the detection of key molecular entities such as non-targeted metabolites, neurotransmitters, amino acids, short-chain fatty acids, and TMAO. Coupled with bioinformatics tools like GO and KEGG, this approach facilitates a systematic dissection and in-depth exploration spanning from tissue phenotypes to molecular mechanisms, thereby providing a high-throughput, multidimensional, end-to-end integrated technological solution for life sciences research.

Experiment Name	Content	Explanation
Metabolite detection	Non-targeted metabolomics	Sample extraction + instrument analysis + data interpretation
	Short-chain fatty acid assay (SCFA)	Sample extraction + instrument analysis + data interpretation
	Qualitative and quantitative analysis of amino acids	Sample extraction + instrument analysis + data interpretation
	Quantitative analysis of neurotransmitters	Sample extraction + instrument analysis + data interpretation
	Quantification of TMAO and its analogs	Sample extraction + instrument analysis + data interpretation
	Flavonoid Quantification	Sample extraction + instrument analysis + data interpretation
Proteomics experiment	label free	Sample extraction + instrument analysis + data interpretation
	Label-based quantitative proteomics (starting from 6 samples)	Sample extraction + instrument analysis + data interpretation
	DAY	Sample extraction + instrument analysis + data interpretation
	Solution-phase mass spectrometry identification	GO and KEGG analyses can be provided.
Gene transcriptome analysis	mRNA sequencing	6G, including extraction, standard + advanced analytics
	m6A RNA methylation
	Single-cell sequencing	10x 120G data volume
	Metagenome	10G+ Standard Analysis + Advanced Analysis
	16s detection	At least 350,000 sequences
	m7G RNA methylation