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Cellular and Molecular Protein Experiment

Aniphe BioLab has established a standardized in vitro research platform covering cellular, molecular, and protein studies. Services include primary cell isolation, cell culture, transfection, stable cell line generation, and functional assays for cell viability, apoptosis, migration, and invasion.The platform integrates flow cytometry, protein interaction and expression analysis (WB, ELISA, Co-IP, and ChIP), RNA extraction, qPCR, and various RNA analyses to enable systematic investigation at the gene and protein levels. By combining complementary technologies, Aniphe provides integrated validation from cellular phenotypes to molecular mechanisms, supporting mechanistic studies, target validation, and drug efficacy evaluation.

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Service Content

Experiment Name Content Explanation
Cell isolation and culture Primary cell isolation Needs assessment
Cell culture Includes culture medium and serum
Anaerobic cell culture Needs assessment
Cell transfection Patch clamp Current magnitude, changes in gating characteristics
Plasmid Construction + Cell Transfection (Transient) DNA, small-molecule oligonucleotides, or siRNA
Stable Transfection (Construction of Stable Cell Lines) Includes lentivirus, DNA, small-molecule oligonucleotides, or siRNA.
Adenovirus Packaging and Infection Needs assessment
Cellular Function Assay Co-culture in a small chamber Needs assessment
Chemical dosing treatment Needs assessment
Cell counting Trypan blue staining
CCK-8 assay Medicines, cells
Cell scratch assay Contains reagent
Transwell migration Contains reagent
Transwell invasion Contains reagent
Cell culture slide Contains reagent
Cell smear Contains reagent
TUNEL assay for apoptosis Contains reagent
Comet Experiment No analysis included
Live/dead cell staining PI/Hoechst staining, observed by fluorescence microscopy.
Flow cytometry Fluorescent detection of membrane potential Contains reagent
Cell Cycle (PI) Includes reagents and flow cytometry analysis
Cell apoptosis (Annexin V-FITC/PI) Includes reagents and flow cytometry analysis
Reactive oxygen species (ROS) Includes reagents and flow cytometry analysis
Mitochondrial Membrane Potential (JC-1) Includes reagents and flow cytometry analysis
Surface Marker Analysis Indicator antibodies must be provided.
Intracellular Marker Analysis Indicator antibodies must be provided.
Cellular marker membrane disruption Needs assessment
Flow sorting Indicator antibodies must be provided.
Magnetic bead sorting Single-Label Positive Selection (LS) Magnetic beads are required.
Single-Label Negative Selection (LD) Magnetic beads and separation columns must be provided.
Dual-standard sorting Magnetic beads are required.
Molecular protein immunoassay ELISA assay at 48 hours Includes kit fee
ELISA assay 96t Includes kit fee
Western blotting Antibodies are required.
WB/PCR Special Sample Protein/DNA Extraction A separate handling fee will be charged for samples that are difficult to process.
BCA assay Protein concentration determination by the BCA method
Co-IP experiment Contains magnetic beads, does not contain antibodies.
ChIP assay Reagents must be supplied by the customer; samples must be fresh.
Pull-Down Antibodies are required.
EMSA assay (gel shift assay) Does not include the sample extraction fee.
Enzyme profiling  
Fully automated biochemical analyzer testing Standard items; quotation for special items
Primer Design and Synthesis Primer specificity validation
RNA extraction and reverse transcription Trizol
Conventional gene qPCR (SYBR Green dye–based assay, run on a real-time PCR instrument) SYBR Green dye method
miRNA, Lnc, and circRNA qPCR analysis using the SYBR Green dye method SYBR Green dye method
  Exosome Isolation and Identification Includes extraction, Western blotting (WB), NTA, and scanning electron microscopy; a single extraction yields 1 mL, with additional charges for volume expansion.
Mycoplasma detection  
Mycoplasma clearance Two-week removal period; if thorough removal cannot be achieved despite trying various methods, standard fees will apply.