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Cellular and Molecular Protein Experiment

Aniphe BioLab Experimental Service Category List
Experiment Name Content Explanation
Cellular and Molecular Protein Experiment
Cell isolation and culture Primary Isolation and Identification of Cells Needs assessment
Cell line culture Includes culture medium and serum
Cell transfection Patch clamp Current magnitude, changes in gating characteristics
Transient transfection/Stable transfection DNA, small-molecule oligonucleotides, or siRNA
Construction and Validation of Stable Cell Lines DNA, small-molecule oligonucleotides, or siRNA
Lentivirus Packaging and Infection Cells are required.
Cellular drug treatment Cell co-culture Preferably, adherent cells and suspension cells.
Chemical dosing treatment The drug concentration and duration must be clearly specified.
Cellular Function Assay MTT and CCK8 assays Medications and cells are required.
Cell growth curve Cells are required.
Cell scratch assay Cells are required.
Transwell There are two types: invasion and metastasis.
Cell culture slides, smears Specifications must be clarified.
TUNEL apoptosis assay Cells are required.
Flow cytometry Cell Cycle (PI) Includes kit costs; cells must be provided.
Apoptosis
(Annexin V-FITC/EGFP/PE/APC) 		Includes kit costs; cells must be provided.
Reactive oxygen species (ROS) Includes kit costs; cells must be provided.
Mitochondrial Membrane Potential (JC-1) Fresh, viable cells are required.
Surface Marker Analysis Indicator antibodies must be provided.
Intracellular Marker Analysis Indicator antibodies must be provided.
Flow cytometry sorting Indicator antibodies must be provided.
Molecular protein immunoassay 1 ELISA assay, 48 wells/96 wells Includes kit fee
Western blotting Antibodies are required.
Total protein extraction A separate handling fee will be charged for samples that are difficult to process.
1 Nucleoprotein/Membrane Protein Extraction Includes kit fee
Co-IP experiment Antibodies are required.
1 ChIP assay Reagents must be supplied by the customer, and samples must be fresh.
Pull-down Antibodies are required.
EMSA assay (gel shift assay) Does not include the sample extraction fee.
Enzyme profiling  
Single-cell suspension (grinding method) Grinding method, digestion method
Primer Design and Validation Primer specificity validation
1 RNA Extraction and Reverse Transcription Trizol
Dye-based qPCR assay for instrument-based detection SYBR Green dye method
miRNA, Lnc, and circRNA on-machine detection SYBR Green dye method