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MTT and CCK8 assays
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MTT and CCK8 assays

MTT assay

Also known as the MTT assay, it is a method used to assess cell viability and proliferation.

The underlying principle of this assay is that succinate dehydrogenase in the mitochondria of viable cells can reduce exogenous MTT to an insoluble blue‑purple formazan precipitate, which accumulates within the cells; dead cells lack this enzymatic activity. Dimethyl sulfoxide (DMSO) dissolves the formazan, and its absorbance is measured at 490 nm using a microplate reader, providing an indirect indication of the number of viable cells. Within a certain cell‑number range, the amount of MTT‑derived formazan formed is directly proportional to the cell count. This method has been widely employed for assessing the biological activity of various bioactive factors, high‑throughput screening of anticancer drugs, cytotoxicity assays, and evaluation of tumor radiosensitivity, among other applications. It is characterized by high sensitivity and cost‑effectiveness.

 

CCK-8

The Cell Counting Kit‑8 (CCK‑8) reagent is suitable for accurate assays of cell proliferation and cytotoxicity. Its underlying principle is that the kit contains WST‑8, which, in the presence of the electron‑coupling agent 1‑methoxy‑5‑nitro‑2‑phenyltetrazolium bromide (1‑MethoxyPMS), is reduced by dehydrogenases to a highly water‑soluble yellow formazan dye. The amount of formazan produced is directly proportional to the number of viable cells; thus, this property can be exploited for direct cytotoxicity analysis.

 

Purpose : Drug screening, cell proliferation assays, cytotoxicity assays, and drug sensitivity testing.

 

Aniphe BioLab Service Workflow

 

1. Cell Culture and Seeding 96 Orifice plate

2. Administer medication and perform other treatments.

3. Perform according to customer requirements. CCK8 Testing experiment

4. Data Statistical Analysis and Writing of Experimental Reports

 

Aniphe BioLab customer provided :

 

Providing biologics such as cells and drugs in good condition.

 

Aniphe BioLab supply :

 

Raw data and statistical analysis results, complete experimental report

 

Aniphe BioLab Assurance :

 

Good repeatability, accurate analysis, and detailed reporting.

 

Aniphe BioLabl delivery time :

 

3-7 Weekday