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Transient transfection/Stable transfection
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Transient transfection/Stable transfection

Cell transfection refers to to will Exogenous molecules such as Techniques for delivering DNA, RNA, and other biomolecules into eukaryotic cells have become standard tools for studying and manipulating gene function in eukaryotes. They are widely employed in biological experiments involving gene function, regulation of gene expression, mutation analysis, and protein production.

 
Cell transfection Technical protocols can be broadly categorized into two types: transient transfection and stable transfection.


Transient transfection ：

 

Outside Source DNA/RNA does not integrate into the host chromosome; therefore, a single host cell can harbor multiple copies, resulting in high-level expression. However, this state typically lasts only a few days and is commonly used for analyzing promoters and other regulatory elements.

 

Service Process:


1. The customer provides the relevant cell line and plasmid.
2. Cell Culture and Plasmid Transfection
3. Detection of the target protein by immunofluorescence or Western blotting
4. Prepare the experimental report


Stable transfection : Exogenous DNA can either integrate into the host chromosome or exist as a free extrachromosomal element (episome).


Stable transfection


1. Plasmid transfection of cells
2. Selection under pressure using antibiotics such as G418
3. Detection of the target protein by immunofluorescence or Western blotting
4. Cell Expansion, Cryopreservation, and Preparation of the Experimental Report

 

 

Service Process:


1. The customer provides the relevant cell line and plasmid.
2. Cell Culture and Plasmid Transfection
3. Detection of the target protein by immunofluorescence or Western blotting
4. Prepare the experimental report