Introduction to Animal Models of Abdominal Aortic Aneurysm

Animal models of abdominal aortic aneurysm (AAA) are essential experimental tools for simulating the pathological processes underlying human AAA. These models typically induce elastic fiber degradation, inflammatory infiltration, and thinning of the vascular media in the aortic wall through methods such as intraluminal infusion of elastase, chronic infusion of angiotensin II via osmotic pumps, or external application of calcium chloride. Such approaches lead to localized dilation of the abdominal aorta. These models can recapitulate key clinical features, including progressive aneurysmal expansion, thrombus formation, and even rupture.

Aniphe Biolaboratory's AAA Animal Model Platform: Service Advantages

Aniphe Biolaboratory abdominal aortic aneurysm (AAA) animal model platform, centered on a standardized modeling system and the capability to precisely simulate multiple pathological stages, provides a stable and reproducible in vivo research foundation for pharmacological efficacy evaluation and mechanistic studies.

	Modeling methods are mature and diverse. Employing mainstream modeling approaches such as local perfusion with elastase, it is adaptable to diverse research objectives.
	The model has high stability. The tumor formation rate and expansion curve are controllable, and key pathological milestones exhibit high reproducibility.
	Pathological features closely resemble clinical findings. It can reliably simulate typical pathological changes, including inflammatory infiltration of the vascular wall, destruction of the elastic lamina, and arterial dilation.
	Multi‑time‑window evaluation system Supports a hierarchical study design spanning from the early stages of disease onset to the late-stage rupture risk window.
	Multi-dimensional detection support Systematic pharmacological efficacy evaluation can be conducted by integrating imaging, histological, and molecular biological markers.

Modeling cycle	1–4 weeks
Laboratory animals	Mouse
Positive drug	Doxycycline (an MMP inhibitor that can suppress aneurysmal dilation), losartan (an AT1 receptor antagonist that inhibits TGF-β and inflammatory signaling), and certain antioxidants (such as proanthocyanidin PGG) are commonly used for mechanistic validation.
Application areas	Pathogenesis of aneurysms (inflammation, extracellular matrix degradation, oxidative stress), pharmacological efficacy evaluation of drugs and molecular targets, biomarker screening (e.g., MMP‑9, IL‑6), and preclinical testing of novel vascular stents and graft materials.

Construction of AAA animal models

Animal models of abdominal aortic aneurysm (AAA) are commonly established using elastase perfusion. The underlying principle involves exposing the abdominal aorta under anesthesia and locally infusing elastase, which selectively degrades elastic fibers in the vascular media, thereby compromising the structural integrity of the vessel wall and inducing progressive dilation. Following surgery, processes such as inflammatory cell infiltration, extracellular matrix degradation, and smooth muscle cell loss occur, ultimately yielding an aneurysm model that closely mimics the pathological features of human AAA and exhibits stable, reproducible characteristics.