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Animal model of sepsis-induced lung injury

Animal model of sepsis-induced lung injury

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Sepsis-associated ARDS
Psychiatric & neurological disease models

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Model Introduction

Model Introduction

Platform Service Advantages

Platform Service Advantages

Model Construction

Model Construction

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Sepsis-associated ARDS

Introduction to Animal Models of Sepsis-Induced Lung Injury

The Ailinfei biological animal model of sepsis‑induced lung injury is constructed based on core pathogenic mechanisms, including dysregulated systemic inflammatory response, immune dysfunction, and disruption of pulmonary microvascular barrier function. It primarily induces systemic infection or endotoxin challenge to mimic the cascade of pulmonary inflammation, alveolar–capillary barrier damage, and pulmonary edema that occur in sepsis. This model accurately reflects the onset and progression of sepsis‑associated acute lung injury (ALI/ARDS).

Service Advantages of the Aniphe BioLablogical Sepsis-Induced Lung Injury Animal Model Platform

To provide a standardized, reproducible in vivo experimental model platform for studying the mechanisms of sepsis‑associated acute lung injury and for evaluating therapeutic efficacy.

Comprehensive model coverage

The model has been built to be mature and stable.

The classic CLP (cecal ligation and puncture) model was employed, yielding a high success rate and robust phenotypic consistency.

Standardization of the modeling process

The pathological phenotype is well-defined and controllable.

It can reliably induce typical ALI/ARDS manifestations, including alveolar structural damage, inflammatory cell infiltration, and pulmonary edema.

Multidimensional Evaluation System

Refinement of the multidimensional evaluation system

The extent of injury was comprehensively assessed by integrating pulmonary histopathology, inflammatory cytokine measurements, and pulmonary function parameters.

Adapted for multi-cycle studies

Adaptable to diverse research needs

Supports research on the mechanisms of acute inflammatory injury and the evaluation of pharmacological interventions across different time windows.

Standardization of Data Delivery

Complete data delivery specifications

Provide raw experimental data, pathology slide images, and systematic analysis results to ensure data traceability and compliance with publication‑grade standards.

Modeling cycle

1–2 weeks

1  Laboratory animals

Mice and rats of SPF grade

1  Positive drug

Common anti-inflammatory and lung-protective agents include dexamethasone, methylprednisolone, edaravone, and ulinastatin.

1  Application areas

It is widely used in studies of the mechanisms underlying sepsis‑associated acute lung injury, investigations into the regulatory networks of inflammatory cytokines, elucidation of the pathogenic mechanisms of pulmonary barrier dysfunction, and evaluation of the therapeutic efficacy of anti‑inflammatory and lung‑protective agents.

Establishment of an Animal Model of Sepsis-Induced Lung Injury

01 After weighing the mice in a quiet environment, they were anesthetized. Once fully anesthetized, the abdominal fur was shaved, the mice were secured on the surgical table, and the exposed skin of the abdomen was disinfected with povidone‑iodine.

02 Maintain aseptic technique, and use a scalpel to sequentially incise the layers of tissue in the mouse’s abdomen until the abdominal cavity is opened; the surgical incision should be approximately 1 cm in length.

03 After locating the distal end of the mouse cecum, gently traction it into view, and ligate it with a sterile suture approximately 1 cm from the cecal tip. (If the distal end contains only a small amount of feces, use blunt forceps to gently compress the cecum, thereby extruding its contents toward the cecal terminus.)

04 Then, using a sterile 10-mL syringe needle, gently puncture the cecal ligation site twice, carefully express a small amount of feces and wipe the area clean, before returning the cecal stump to the mouse’s abdominal cavity.

05 After cleaning the wound, the mouse’s tissues were sequentially sutured layer by layer. Once the mouse had recovered from anesthesia, it was returned to its cage and maintained under stable housing conditions.

Model features: Cecal ligation and puncture in animals is the gold-standard model for reproducing clinical sepsis, particularly gastrointestinal perforation; it accurately reflects the bidirectional changes in immunology and hemodynamics that occur during the progression of sepsis.