Introduction to SA Animal Models

The spontaneous abortion (SA) mouse model is a classic reproductive disease model established through allogeneic pairing, immune intervention, pharmacological induction, or hormonal regulation, and is used to simulate embryonic resorption and miscarriage during early pregnancy. This model effectively recapitulates pathological features such as maternal–fetal immune tolerance imbalance, aberrant expression of inflammatory cytokines, decidual developmental defects, impaired placental function, and abnormal embryonic development, manifesting as elevated embryo resorption rates, reduced fetal numbers, and adverse pregnancy outcomes. The spontaneous abortion mouse model offers advantages including well‑established methodology, high reproducibility, and a clearly defined underlying mechanism.

Advantages of Aniphe BioLab’s SA Animal Model Platform Services

Relying on a well-established animal model platform for reproductive disorders and a standardized pharmacological efficacy evaluation system, Aniphe BioLab offers stable, highly reproducible animal models of spontaneous abortion (SA), providing robust preclinical research support for reproductive medicine and the development of drugs to prevent miscarriage.

	The modeling protocol is well-established. It supports multiple modeling approaches, including classical immune‑mediated spontaneous abortion and drug‑induced spontaneous abortion, with high model success rates and excellent reproducibility.
	The model is stable and reliable. A standardized modeling protocol enables consistent induction of hallmark phenotypes, including increased embryonic resorption, placental developmental abnormalities, and maternal–fetal immune dysregulation.
	Multidimensional evaluation system It provides multiple evaluation parameters, including histopathological analysis, hormone levels, inflammatory cytokines, and immunological assays.
	Supports customized research Modeling protocols, administration routes, gestational time points, and endpoints can be tailored to meet the specific requirements of each study, thereby accommodating diverse research objectives.
	Standardized Quality Management Standardized procedures and quality control throughout the entire process ensure that experimental data are authentic, complete, stable, and traceable.

Modeling cycle	6–8 weeks
Laboratory animals	CBA/J and BALB/c mice
Positive drug	Commonly used progestational agents include dydrogesterone, progesterone, human chorionic gonadotropin (hCG), and aspirin in combination with low-molecular-weight heparin, among others.
Application areas	It is widely employed in research on the pathogenesis of recurrent pregnancy loss, maternal–fetal immune tolerance, placental developmental abnormalities, and endocrine regulation. It is also suitable for preclinical efficacy evaluation of innovative therapies, including tocolytic agents, immunomodulators, traditional Chinese medicines and natural products, and biologics, thereby providing a reliable in vivo experimental platform for reproductive medicine and the study of pregnancy‑related disorders.

Construction of an SA animal model

Using the classic Clark model of recurrent miscarriage in mice, CBA/J females were mated with DBA/2 males to establish a natural abortion model, while CBA/J females mated with BALB/c males served as a control group for normal pregnancy. The model induces embryonic resorption through disruption of maternal–fetal immune tolerance and consistently exhibits hallmark features such as increased embryonic resorption rates, abnormal placental development, and heightened inflammatory immune responses. With advantages including well-established methodology, excellent reproducibility, and high clinical relevance, this model is a standard animal model for investigating the pathogenesis of recurrent spontaneous abortion and for preclinical efficacy evaluation of anti‑abortive therapeutics.