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Animal model of avascular necrosis of the femoral head

Animal model of avascular necrosis of the femoral head

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Avascular necrosis of the femoral head
Bone-related disease model

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Model Introduction

Model Introduction

Platform Service Advantages

Platform Service Advantages

Model Construction

Model Construction

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Avascular necrosis of the femoral head

Introduction to ANFH Animal Models

An animal model of avascular necrosis of the femoral head (ANFH) is established by mimicking localized impairment of blood supply to the femoral head, thereby inducing ischemic necrosis of osteocytes and bone marrow tissue. This leads to pathological changes such as trabecular bone destruction, dysregulated bone remodeling, and structural collapse of the femoral head. The model effectively replicates the disease progression and histological features observed in human ANFH, reflecting key pathophysiological processes including ischemic injury, abnormal bone metabolism, and diminished bone repair capacity. Consequently, it provides a reliable in vivo platform for investigating disease mechanisms and evaluating therapeutic interventions.

ANFH Animal Model Platform Service Advantages of Aniphe BioLab

Leveraging mature animal modeling technologies and a robust pharmacological evaluation system, Aniphe BioLab offers standardized, customized, end-to-end experimental services for research on avascular necrosis of the femoral head (ANFH).

Comprehensive model coverage

The model is stable and reliable.

Standardized operating procedures ensure a high modeling success rate and excellent reproducibility.

Standardization of the modeling process

Bone density testing has been improved.

We offer Micro-CT, histopathology, bone biochemistry, and bone biomechanics assessments.

Multidimensional Evaluation System

Animal Management Regulations

Standardized feeding reduces the influence of exogenous factors on the model.

Adapted for multi-cycle studies

Supports custom research

The modeling cycle, dosing regimen, and experimental design can be flexibly adjusted.

Adapted for multi-cycle studies

Full-process quality is controllable.

End-to-end quality control throughout the modeling process, with complete traceability of raw data.

Adapted for multi-cycle studies

One-stop experimental services

Covering the entire workflow, from model creation and intervention to testing and data analysis.

Modeling cycle

4–6 weeks

1  Laboratory animals

Mice and rats of SPF grade

1  Positive drug

Alendronate sodium, alprostadil, and statins are commonly used as positive controls; they can improve bone microcirculation, inhibit bone resorption, promote bone repair, and maintain bone microarchitecture, making them suitable for model validation and efficacy evaluation.

1  Application areas

It is primarily used for studying the pathogenesis of femoral head avascular necrosis, as well as for preclinical in vivo efficacy evaluation of hip-preserving therapies, bone‑repair–promoting drugs, biologics, stem cell treatments, and tissue‑engineering materials.

Construction of an ANFH animal model

01 Liquid Nitrogen Cryotherapy

Through surgical exposure of the femoral head or femoral neck, local cryotherapy with liquid nitrogen is applied to induce hypothermic injury to the bone’s vasculature and osteocytes, thereby disrupting local blood supply and triggering osteocyte necrosis, which gradually leads to ischemic necrosis of the femoral head. This model features a well-defined necrotic area and stable establishment, effectively mimicking the pathological process of traumatic avascular necrosis of the femoral head (ANFH).

02 Hormonal Method

By repeatedly administering high doses of glucocorticoids, such as methylprednisolone or dexamethasone, this approach induces disturbances in lipid metabolism, impairment of intraosseous microcirculation, and osteocyte apoptosis, ultimately leading to ischemic necrosis of the femoral head. This method is simple to perform and exhibits good reproducibility, effectively mimicking the pathogenesis and progression of corticosteroid‑induced ANFH observed in clinical settings, and is widely employed for pharmacological efficacy evaluation and mechanistic studies.