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Cell scratch assay
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Cell scratch assay

Principle of the cell scratch assay:

 

When the cells have grown to a certain stage, an artificial pathway is created… “Wound” refers to a scratch, and the cells surrounding the wound gradually migrate into it, leading to its closure—this constitutes a very straightforward method for assessing cell motility. The wound‑healing assay is low‑cost and can be used to evaluate the invasive and metastatic potential of adherent tumor cells. It is worth noting that this technique is applicable to a relatively limited range of cell types, typically epithelial and fibroblast‑like cells. Furthermore, using serum‑free medium in the wound‑healing assay allows one to disregard the effects of cell proliferation and serum components on the experiment; however, because the overall signaling pathways within the cells are downregulated, the rate of cell migration will be significantly reduced.

Service Process


1. Cell Culture and Scratch Assay
2. Serum-free and low-serum culture
3. Cell Observation and Imaging
4. Calculating Mobility and Writing the Experimental Report


Customer provided:


1. Provide biologics such as cells and drugs in good condition.
2. Detailed experimental design for the cell scratch assay, including drug concentration and incubation time, etc.


Aniphe BioLab provides:

 

Raw data and statistical analysis results, complete experimental report


Quality Assurance:


Accurate analysis, detailed reporting


Delivery time:


3–7 business days