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Primary Isolation and Identification of Cells
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Primary Isolation and Identification of Cells

Primary cell isolation and culture

 

Service Overview:


Primary cells: Cells that are derived from embryos, tissues or organs, or peripheral blood and prepared through specialized isolation techniques are referred to as primary cells.
Primary cell isolation and culture: Tissue is excised from the animal body and treated with various enzymes. The process involves treatment with trypsin or type II collagenase, chelating agents (commonly EDTA), or mechanical dissociation to obtain single cells, followed by culture in an appropriate medium to support cell survival, growth, and proliferation. High cell purity: primary target cells can be obtained with a purity exceeding 95%; robust cell viability: P0–P3 passages are provided, with a viability greater than 80% and free from contamination; multiple assay options: flow cytometry, immunological assays, real-time PCR, Western blotting, and other methods are available upon request.


Service Process:


1. Collection of primary cells
2. Isolation, culture, and identification of primary cells
3. Passage and Preservation of Primary Cells

Customer provided:


1. The customer must provide a sufficient quantity of fresh, sterile tissue samples and specify the culture conditions for the primary cells.
2. The primary antibody or special staining reagent required for primary cell identification must be provided by the customer or procured on their behalf by us.


Aniphe BioLab provides:


1. One vial of cryopreserved cells or cells in the logarithmic growth phase (within passage P3).
2. One complete experimental report (including cell culture conditions, cell images, and identification results)


Quality Assurance:


The cells are in good condition and free from contamination.


Delivery time:


10–30 business days