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Liver cancer animal model

Liver cancer animal model

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Liver cancer
Digestive system models

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Model Introduction

Model Introduction

Platform Service Advantages

Platform Service Advantages

Experimental results

Experimental results

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Liver cancer

Introduction to Animal Models of Hepatocellular Carcinoma

Animal models of hepatocellular carcinoma can be established through chemical induction, orthotopic or subcutaneous tumor cell implantation, transgenic approaches, and other methods. Following model establishment, the animals progressively develop hepatic tissue lesions and tumor nodule formation, accompanied by abnormal liver enzyme levels, declining liver function, ascites, weight loss, and jaundice, thereby accurately mimicking the pathological progression and clinical manifestations of human hepatocellular carcinoma.

Service Advantages of the Aniphe BioLab Liver Cancer Animal Model Platform

Our platform offers a comprehensive range of hepatocellular carcinoma animal models and mature, well‑established technologies, capable of meeting diverse research needs, including mechanistic studies, drug efficacy evaluation, and new‑drug development submissions.

Comprehensive model coverage

A wide variety of modeling types are available.

It encompasses a variety of classic models, including chemically induced, cell‑inoculated, and transgenic systems, and is suitable for diverse research directions.

Standardization of the modeling process

High clinical relevance

The model can recapitulate hepatic tumor proliferation, tissue injury, and associated complications, closely mirroring the clinical course of human hepatocellular carcinoma.

Multidimensional Evaluation System

The model exhibits strong stability.

High modeling success rate, minimal inter-group variability, and excellent experimental reproducibility ensure data accuracy and reliability.

Adapted for multi-cycle studies

Standardized Quality Control System

SPF-grade animal care and management are employed, with continuous real-time monitoring of animal health throughout the study; experimental data are fully traceable.

Standardization of Data Delivery

Comprehensive testing dimensions

It can perform a wide range of assays and analyses, including histopathological sectioning, biochemical profiling, imaging studies, and molecular biology techniques.

Standardization of Data Delivery

One-stop, end-to-end service

We offer an integrated service encompassing model generation, animal husbandry and observation, sample collection, and biomarker analysis, efficiently supporting the advancement of research projects.

Modeling cycle

2–18 weeks

1  Laboratory animals

Mice and rats of SPF grade

1  Positive drug

Sorafenib and lenvatinib, owing to their broad clinical use and consistent efficacy, are the most commonly employed positive control agents for evaluating the therapeutic effectiveness of anti‑hepatocellular carcinoma drugs; they can effectively inhibit tumor growth, angiogenesis, and metastasis.

1  Application areas

It is widely employed in areas such as antitumor drug screening and efficacy evaluation, investigation of the mechanisms underlying hepatocarcinogenesis and tumor progression, studies of the tumor microenvironment and angiogenesis, assessment of immunotherapy and combination‑therapy strategies, research on drug‑resistance mechanisms, and biomarker discovery, thereby providing a critical in vivo experimental platform for basic hepatocellular carcinoma research and the development of innovative therapeutics.

Experimental results

Construction of the MASH animal model

01 DEN+CCL4 chemically induced model: Combined, stepwise administration of the two agents leads to progressive hepatic injury and fibrosis, culminating in the development of primary hepatocellular carcinoma; multiple tumor nodules are observed in the liver.

02 CDX/PDX orthotopic model: Liver cancer cells or patient tumor tissue are implanted into the liver to mimic in situ tumor growth and invasion.

03 Liver metastasis model: Cells are inoculated via the intravenous route, the spleen, and other pathways to establish tumor liver metastatic lesions.

Model features: A variety of modeling approaches are available, all of which are among the most widely used in current research, delivering superior performance and closely mirroring clinical conditions.

Liver tissue

As evidenced by the scale, the tumor nodules vary in size from a few millimeters and are widely distributed throughout the liver lobes, clearly demonstrating the model’s uniformity and typicality, thereby faithfully replicating the gross pathological features of advanced hepatocellular carcinoma.

 

 

HE staining results

In normal mouse liver tissue, cell outlines are well-defined, nuclei are round and centrally located, and the overall architectural morphology is typical. In contrast, in hepatic tumor tissue, cells are arranged in a disorganized manner, with poorly defined nuclei; vacuolar degenerative changes are evident, and the architecture consists of irregular cellular aggregates, consistent with the structural features of neoplastic tissue.