Introduction to the EAM Animal Model

Idiopathic inflammatory myopathies are autoimmune inflammatory myopathies of unknown etiology, primarily affecting striated muscle. Experimental autoimmune myositis shares many pathological features with human polymyositis and dermatomyositis.

EAM Animal Model Platform Service Advantages of Aniphe BioLab

Our platform boasts mature, standardized protocols for establishing the experimental autoimmune myositis (EAM) model, capable of meeting the needs of diverse research studies and new drug development.

	Model phenotype stability Muscle inflammation, decreased muscle strength, and elevated serum muscle enzymes were all significantly and consistently observed, with a high modeling success rate and excellent experimental reproducibility.
	End-to-end standardized management and control Animals are housed under SPF‑grade conditions, with regular monitoring of activity levels and biochemical parameters, ensuring full traceability of experimental data throughout the study.
	The testing system has been improved. It encompasses multi-dimensional assessment parameters, including muscle enzyme biochemistry, muscle histopathology, inflammatory cytokines, and immune markers.
	The solution is flexible and customizable. The immunization dose, modeling cycle, and intervention duration can be adjusted as needed to accommodate different experimental designs.
	One-stop comprehensive services End-to-end closed-loop delivery spanning model generation, animal care, and sample analysis, supporting both basic research and IND‑submission projects.

Modeling cycle	6–8 weeks
Laboratory animals	C57BL/6J male mice
Positive drug	Glucocorticoids such as prednisone and dexamethasone can effectively suppress inflammatory responses and ameliorate muscle injury.
Application areas	It is widely used in studies of the pathogenesis of autoimmune myopathies, immune‑inflammatory regulation, the mechanisms of muscle injury and repair, inflammatory cell infiltration and cytokine networks, as well as in the evaluation of the efficacy and mechanisms of action of glucocorticoids, immunosuppressants, biologics, traditional Chinese medicines, and novel therapeutics, making it an important experimental model for investigating idiopathic inflammatory myopathies.

Construction of EAM animal models

1. Prepare the antigen and mix it with an adjuvant to form an antigen emulsion.

2. Rats were injected with an antigen emulsion into the plantar surface (footpad), while mice received a subcutaneous injection of the antigen emulsion on the dorsal side of the spine. A second injection of pertussis toxin was administered 48 hours later.

3. The day of injection is designated as Day 0; body weight and clinical neurological scores (according to the Kono scoring system) are recorded daily until 30 days post‑immunization.