Introduction to the AC Animal Model

Allergic conjunctivitis (AC) is an IgE‑mediated type I hypersensitivity disorder of the ocular surface and one of the most common allergic eye diseases in clinical practice. It primarily manifests as ocular pruritus, conjunctival hyperemia, edema, tearing, and inflammatory cell infiltration. Its pathogenesis involves mast cell degranulation, eosinophilic infiltration, and aberrant activation of Th2 immune responses. Animal models of AC are typically established by systemic sensitization with ovalbumin (OVA) or other allergens, followed by local challenge, which effectively recapitulate the immunological features and pathological changes observed in human allergic conjunctivitis, including elevated IgE levels, increased release of pro‑inflammatory cytokines, and inflammatory responses in the conjunctival tissue.

Service Advantages of Aniphe BioLab’s AC Animal Model Platform

Relying on a well-established ophthalmic disease model platform and a standardized experimental system, Aniphe BioLab offers stable, highly reproducible animal models of allergic conjunctivitis (AC), supporting the development of anti-allergic ophthalmic therapeutics.

	Mature modeling system A classic allergen‑induction protocol was employed, yielding a stable model with a high success rate and effectively mimicking the pathological features of clinical asthma.
	Multidimensional Efficacy Evaluation It supports the assessment of ocular symptom scores, histopathology, inflammatory cytokines, IgE levels, and other relevant biomarkers, enabling a comprehensive evaluation of drug efficacy.
	Standardized Quality Control End-to-end SOP management and QA quality control ensure model consistency and the authenticity and traceability of experimental data.
	Customized Experimental Services Supports diverse administration routes and customized study protocols, meeting the preclinical evaluation needs of anti-allergic drugs and ophthalmic formulations.

Modeling cycle	2-3 weeks
Laboratory animals	Mice and rats of SPF grade
Positive drug	Commonly used positive drugs mainly include dexamethasone, olopatadine, and ketotifen.
Application areas	It is widely used in research on the pathogenesis of allergic ocular diseases and in the evaluation of the efficacy of antihistamines, glucocorticoids, immunomodulators, and novel ophthalmic formulations. It is also suitable for studying the mechanisms of immune‑mediated inflammation on the ocular surface, screening biologic agents, and conducting preclinical in vivo assessments of innovative anti‑allergic drugs.

Construction of an AC animal model

An allergic conjunctivitis model was established using a 5% ovalbumin (OVA)–phosphate-buffered saline (PBS) solution. First, systemic sensitization was induced by intraperitoneal injection of OVA emulsified with an adjuvant, thereby establishing an allergic state in the organism. Subsequently, following completion of sensitization, OVA–PBS solution was instilled into both conjunctival sacs for local challenge; repeated stimulation over a defined period elicited typical features of allergic conjunctivitis, including conjunctival hyperemia, edema, lacrimation, ocular scratching, and inflammatory cell infiltration, thus establishing a stable animal model of allergic conjunctivitis. This method is well‑established and highly reproducible, effectively mimicking the pathological hallmarks of IgE‑mediated allergic conjunctivitis observed in clinical settings, and is widely employed for preclinical efficacy evaluation of anti‑allergic ophthalmic agents.