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Animal model of hepatic ischemia-reperfusion injury (IRI)

Animal model of hepatic ischemia-reperfusion injury (IRI)

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Hepatic ischemia-reperfusion injury
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Model Introduction

Model Introduction

Platform Service Advantages

Platform Service Advantages

Model Construction

Model Construction

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Hepatic ischemia-reperfusion injury

Introduction to IRI Animal Models

The liver ischemia-reperfusion injury (IRI) animal model is used to simulate the tissue damage that occurs during the restoration of blood flow following brief hepatic ischemia, mediated by oxidative stress and inflammatory responses. It is widely employed in research on liver transplantation, hepatic resection, and shock‑related liver injury.

The model typically involves surgically occluding the hepatic portal vein and the hepatic artery to induce ischemia—either partial or global—for a defined period (e.g., 30–90 minutes), followed by reperfusion upon removal of the occlusion. This process triggers hepatocellular injury, microcirculatory dysfunction, and an inflammatory cascade. Following model establishment, marked elevations in ALT and AST levels, hepatic tissue necrosis, and neutrophil infiltration are observed, accompanied by increased reactive oxygen species production and the release of pro-inflammatory cytokines.

Advantages of Aniphe BioLab’s IRI Animal Model Platform Services

Aniphe Biolaboratory Animal Model Platform for Hepatic Ischemia-Reperfusion Injury (IRI) is centered on robust ischemia control and reproducible reperfusion‑induced injury, providing a reliable in vivo model system for investigating the mechanisms of liver transplantation–related injury and for evaluating hepatoprotective agents.

Comprehensive model coverage

Ischemia duration is precisely controllable.

The duration of ischemia can be tailored to the research requirements (e.g., 30–90 min), ensuring consistent injury gradients.
Standardization of the modeling process

Reperfusion response is stable.

Standardize reperfusion protocols to ensure the reproducible induction of oxidative stress and inflammatory responses.
Multidimensional Evaluation System

Pathological features closely resemble clinical findings.

It can stably reproduce the typical features of hepatocellular necrosis, microcirculatory dysfunction, and inflammatory cell infiltration.
Adapted for multi-cycle studies

Oxidative stress markers are well-defined.

It can induce an increase in ROS levels and disrupt the antioxidant system, highlighting the need for studies on adaptation mechanisms.
Standardization of Data Delivery

The multi-dimensional evaluation system has been refined.

Systematic evaluation is achieved by integrating ALT/AST levels, biochemical parameters, and histopathological analysis.
Modeling cycle

1 week

1  Laboratory animals

C57BL/6 male mice

1  Positive drug

N-acetylcysteine (antioxidant), dexamethasone (anti-inflammatory), melatonin (scavenges oxygen free radicals), adenosine A2A receptor agonists, and others are used to assess the model’s responsiveness to interventions targeting oxidative stress and inflammation.

1  Application areas

Pathogenesis of hepatic ischemia-reperfusion injury (oxidative stress, sterile inflammation, mitochondrial damage); screening of hepatoprotective agents; evaluation of perioperative protective strategies in hepatectomy and liver transplantation; research on the effects of ischemic preconditioning and postconditioning interventions.

Construction of IRI animal models

Laparotomy: Make a midline incision in the abdomen to expose the liver. Carefully dissect the connective tissue surrounding the liver, taking care to avoid injury to adjacent organs.

Hepatic portal occlusion: A non‑invasive vascular clamp is used to occlude the hepatic portal, including the portal vein and the hepatic artery, thereby mimicking an ischemic state. The duration of occlusion typically ranges from 30 minutes to 1 hour, with the exact timing adjusted according to experimental requirements.

Reperfusion: Remove the vascular clamps to restore hepatic blood flow, thereby mimicking the reperfusion state. The reperfusion period typically ranges from 1 hour to several hours, with the exact duration adjusted according to experimental requirements.

Closure of the abdomen: The abdominal incision is sutured, ensuring there is no bleeding and no risk of infection. Antibiotics are administered for infection prophylaxis.

Photos of key steps in model creation