All

All
Insight
Research Services
Preclinical CRO
Animal Resource Bank

Latest News

Focusing on cutting-edge technologies and research advancements,
sharing Aniphe BioLab’s achievements in scientific innovation, Professional insights
and practical experience in the fields of laboratory animals and preclinical research.

Publish an article

2026-10-29

Naming Genetically Engineered Mice (Part II): From Being Understandable to Being Written Correctly

2026-10-29

How should a multi-disease composite mouse model be constructed? Let’s take a look at the CKM approach.

2026-08-12

2026 CKM Guidelines Released: Chronic Diseases Enter the Era of Integrated Cardiorenal-Metabolic Management—It’s Time to Rethink Animal Models

2026-08-12

A Guide to Safe Mouse Handling | Manual on Mouse Transport Protocols and Troubleshooting

45-day ultra‑fast propagation! IVF‑ET assisted reproductive technology helps your little mouse get a head start!

When faced with these challenges in natural breeding, IVF‑ET—In Vitro Fertilization and Embryo Transfer—can help you quickly and easily generate genetically diverse, age‑synchronized mouse offspring. With mouse breeding accelerated by up to 50%, your paper will be one step ahead!

What is IVF-ET?

IVF-ET is an assisted reproductive technology commonly used in the breeding of genetically engineered mice and in bio‑purification processes.

| Principle:

Mice are induced to ovulate, yielding a large number of oocytes that are then fertilized with sperm. Under controlled in vitro conditions, the fertilization process is completed, and the resulting embryos are transferred into a surrogate mother, where they continue to develop until birth.

| IVF-ET Experimental Procedure

Hormonal superovulation → sperm collection + oocyte retrieval → fertilization + recipient preparation → embryo transfer.

Image source: Aniphe BioLab

| Traditional Mating and Breeding vs. Assisted Reproduction

Example 1:

Image source: Aniphe BioLab

Explanation:

a. On average, 5 female mice can produce 30–40 offspring;

b. One male mouse can produce up to 600 offspring;

c. In the offspring, males outnumber females by 31.3%.

(The above data are derived from the Animal Center’s previous statistical analysis of rapid, large-scale IVF propagation (n=7) and are provided for reference only.)

Example 2: Comparison of the time required to obtain 4-week-old homozygous offspring via different reproductive methods

Image source: Aniphe BioLab

Note: Two pairs of heterozygous single-gene mice were bred to produce homozygous offspring of the same batch at 4 weeks of age.

①12 individuals: naturally bred > 22 weeks; IVF, 16–18 weeks;

②36 individuals: naturally bred > 32 weeks; IVF, 18–22 weeks.

③72 individuals: naturally bred for more than 40 weeks; IVF‑derived for 20–24 weeks.

| Advantages of Assisted Reproduction:

0 1

One to two genotype‑correct male mice can produce at least 50 or more offspring within 45 days.

0 2

If the oocyte donor is homozygous, superovulation and IVF can be performed at 3–5 weeks of age, thereby saving time while waiting for sexual maturity.

0 3

The offspring mice from the same litter share a uniform birth date, typically differing by no more than 2–3 days, thereby effectively minimizing experimental variability attributable to age differences and ensuring the accuracy and reliability of the experimental data.

0 4

The pseudopregnant recipient mice meet SPF standards, ensuring the quality of their offspring.

| Successful Cases of Aiweifei Biotechnology

Nxxx homozygous mouse breeding

Project Background: Older male and female rats have reduced fertility, making it difficult to achieve the desired population through natural reproduction.

Using assisted reproductive technologies: Using 10 aged homozygous female mice and 1 aged homozygous male mouse, we successfully obtained 51 homozygous target mice for the client.

APC-Min mice with colorectal cancer

Project Background: The experiment requires a large number of laboratory mice that are from the same batch, of the same age, and of the same sex.

Using assisted reproductive technology : Using four male mice, 321 offspring were successfully bred.

A homozygous mouse of a certain valuable strain

Project Background: Microbial contamination in laboratory mice requires purification and amplification.

Using assisted reproductive technologies: The company selected nine 4-week-old female mice of this strain and two 12-week-old male mice, successfully bred 59 offspring, thereby achieving both strain purification and mouse colony expansion.

Summary

During the implementation of IVF‑based embryo expansion, strict quality control is exercised over mouse strain, reagent storage conditions, capacitation duration, mouse lineage, and the research experience of the laboratory personnel.

Aniphe BioLab boasts mature IVF expansion technology and a team with nearly ten years of experience in mouse IVF, enabling rapid production of large numbers of age‑matched, high‑quality mice within a short timeframe. We also offer strain preservation, bio‑cleaning, and other related services. We welcome all clients to contact us for more information!