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2026-10-29

Naming Genetically Engineered Mice (Part II): From Being Understandable to Being Written Correctly

2026-10-29

How should a multi-disease composite mouse model be constructed? Let’s take a look at the CKM approach.

2026-08-12

2026 CKM Guidelines Released: Chronic Diseases Enter the Era of Integrated Cardiorenal-Metabolic Management—It’s Time to Rethink Animal Models

2026-08-12

A Guide to Safe Mouse Handling | Manual on Mouse Transport Protocols and Troubleshooting

An efficient IVF‑ET assisted reproductive technology platform, enabling rapid colony expansion in just 45 days and fully meeting the needs of research laboratories for mice of all strains.

When encountering these challenges in natural reproduction, IVF-ET In vitro fertilization - Embryo transfer (In Vitro Fertilization-embryo Transfer) Technology enables you to obtain genetically diverse, age-synchronized mouse offspring quickly and easily. Mouse breeding is accelerated by a factor of two, allowing you to… paper One step ahead!

What is IVF-ET?

IVF-ET is an assisted reproductive technology commonly used in the breeding of genetically engineered mice and in bio‑purification processes.

| Principle:

Mice are induced to ovulate, yielding a large number of oocytes that are then fertilized with sperm. Under controlled in vitro conditions, the fertilization process is completed, and the resulting embryos are transferred into a surrogate mother, where they continue to develop until birth.

| IVF-ET Experimental Procedure

Hormonal superovulation →Sperm collection + egg retrieval → Fertilization + recipient preparation → Embryo transfer.

Image source: Aniphe BioLab

| Traditional Mating and Breeding vs. Assisted Reproduction

Example 1：

Explanation:

a. On average, 5 female mice can produce 30–40 offspring;

b. One male mouse can produce up to 600 offspring;

c. In the offspring, males outnumber females by 31.3%.

(The above data are sourced from the Animal Center’s previous… IVF rapid large-scale propagation data statistics (n=7), for reference only.

Example 2: Obtained through different reproductive methods Comparison of time points in 4-week-old homozygous offspring

Explanation: Two pairs of heterozygous single-gene mice were bred to produce homozygous offspring of the same batch at 4 weeks of age.

①12 Only: Natural reproduction > 22 Week; IVF 16-18 Week;

②36 Only: Natural reproduction > 32 Week, IVF18-22 Week;

③72 Only: Natural reproduction exceeds 40 Week, IVF20-24 Week.

| Advantages of Assisted Reproduction:

01, One to two genotype‑correct male mice can produce at least 50 or more offspring within 45 days.

02, If the egg donor is homozygous, then in… 3-5 Superovulation can be performed as early as the specified week of age, and… IVF , saving the time required for sexual maturity;

03、 The offspring mice from the same litter share the same birth date, typically differing by within Within 2–3 days, experimental errors arising from age-related variability were effectively minimized, thereby ensuring the accuracy and reliability of the experimental data.

04、 Pseudopregnant female mice (recipients) meet the criteria. SPF Grade, with guaranteed offspring quality.

| Successful Cases of Aiweifei Biotechnology

Nxxx Inbred mouse breeding

Project Background: Older male and female rats have reduced fertility, making it difficult to achieve the desired population through natural breeding.

Using assisted reproductive technologies: Use 10 Only aged homozygous female mice, 1 Only aged homozygous male mice were successfully obtained for the client. 51 Only homozygous target mice.

Colorectal cancer APC-Min Mouse

Project Background: The experiment requires a large number of laboratory mice that are from the same batch, of the same age group, and of the same sex.

Using assisted reproductive technology : Use 4 Only male mice successfully bred 321 Only the offspring mice.

A homozygous mouse of a certain valuable strain

Project Background: Microbial contamination in laboratory mice requires purification and amplification.

Using assisted reproductive technologies: Company selection 9 only 4 Female rats of this strain at the age of weeks and 2 only 12 Male mice of a certain age successfully bred. 59 Only the offspring are used, achieving purification while simultaneously expanding the mouse colony.

Summary

IVF During the implementation of proliferation techniques, strict controls are exercised over mouse quality, the storage conditions of reagents, the timing of capacitation, mouse strain, and the research experience of the operators.

Aniphe BioLab possesses mature… IVF Propagation techniques and near 10 a one-year-old mouse IVF Our experienced team can help you rapidly obtain a large number of high-quality, age-matched mice in a short period, and we also offer additional services such as strain preservation and bio‑cleaning. We welcome all customers to contact us for inquiries!