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Rat asthma model

　　Model Introduction

　　The purpose of establishing a rat model of asthma is to provide an ideal animal model for the development of new drugs for the diagnosis and treatment of asthma. To investigate the pathogenesis of asthma, numerous animal models have been developed, including mice, rats, guinea pigs, hamsters, dogs, sheep, horses, and monkeys; among these, rats and guinea pigs are the most widely used. In addition to their high reproductive capacity and cost-effectiveness, rat models of asthma exhibit many features that closely resemble those of human asthma. Therefore, it is essential to develop novel asthma therapeutics using the rat asthma model.

　　Modeling method

　　Animal species and strain: SD rats

　　Animal Age: Adult

　　Animal weight: 200–250 g

　　Preparation time: 4–5 weeks, adjustable based on experimental requirements.

　　Preparation method: On day 1, each rat receives a subcutaneous injection of 1 mL of OVA suspension into the medial aspect of the thigh, along with an intraperitoneal injection of 1 mL of inactivated Bordetella pertussis suspension as an adjuvant. On day 8, sensitization is repeated once. Starting on day 15, the rats are placed in glass chambers and, every other day, exposed for 30 minutes to 50 mL of a 2% OVA suspension delivered via an ultrasonic nebulizer at a moderate mist rate, three times per week. The duration of challenge is adjusted according to the experimental objectives, with exposures lasting 1 week, 2 weeks, or 4 weeks or longer.

　　Following OVA aerosol challenge, asthma induction was considered successful if rats exhibited symptoms such as restlessness, choking cough, increased respiratory rate, and sluggishness or recumbency, accompanied by histopathological examination of lung tissue revealing eosinophilic and lymphocytic infiltration, as well as constriction of small airways and small blood vessels.

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